Biotechnology for Sustainable Development to Meet Global Challenges

Abstract

Antimicrobial resistance (AMR) in food-borne pathogens especially Escherichia coli, is of a serious concern in recent years. Extended beta-lactamase resistant (ESBL) resistant E.coli species have recently emerged and have been the source of concern in both human and animal health. This study was conducted to detect the presence of ESBL resistant E.coli in raw chicken meat and bean sprouts in Kota Bharu, Kelantan by using Kirby-Bauer methods for antibiotic sensitivity test (AST) and molecular detection of the ESBL resistance encoding genes by using polymerase chain reaction (PCR). A total of 100 samples comprised of 50 raw chicken meat and 50 bean sprouts were collected and processed microbiologically. Out of 100 food samples that were collected and processed through routine microbiological isolation, 31% (31/100) of E. coli were identified phenotypically.