Simultaneous estimation of linagliptin and resveratrol: development and validation of a uv-spectroscopic stability-indicating method

Nazreen Tabassum; Rizwan Ahamad; Rama K P; Bushra Jabi; Pragati Kumari Gupta; Mohammad Vaseem Ismail; Mohd Mujeeb; Mohd Aqil

doi:10.22376/ijpbs.v16i4.126

Authors

Nazreen Tabassum Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.
Rizwan Ahamad Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.
Rama K P Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.
Bushra Jabi Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.
Pragati Kumari Gupta Department of Zoology, Department of Zoology, Maharani Janki Kunwar College, Bettiah, West Champaran, Bihar-845438, India.
Mohammad Vaseem Ismail School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi– 110062, India.
Mohd Mujeeb Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.
Mohd Aqil Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi – 110062, India.

Abstract

The simultaneous identification and detection of both medications is a challenge when a pharmaceutical product is formulated with a dual or combination drug delivery system. The most straightforward, precise, and dependable technique among the many that are accessible is UV spectroscopy. We have successfully developed and validated the UV Spectroscopy technique for concurrent estimation of Linagliptin (LGT) and Resveratrol (RSV). The percentage recovery of each drug at 50%, 100%, and 150% of the developed simultaneous equation was found to be 50.3% to 49.7%, 100.8% to 101.3%, and 150.3% to 149.7%for LGT and RSV, respectively. The percent relative standard deviation of the LGT and RSV mixture, the intra-day analysis was determined and found to be 1.221% at λ₁ and 1.162% at λ₂, respectively. In the inter-day study, % RSD was determined to be 1.136% at λ₁ and 1.393% atλ_2, respectively. Based on the data, we conclude that our approach is exact and accurate. Through the use of forced deterioration testing in a stability study, we have further verified our approach. Both of our medications were shown to breakdown in oxidative, alkaline, and acidic degradation conditions. Thermal and photolytic degradation studies were also conducted. Based on the findings, it was determined that our simultaneous estimation UV Spectroscopy approach is dependable and can open up new research avenues.

Keywords:

UV spectroscopy, Force degradation study, Simultaneous estimation, Linagliptin, Resveratrol, Stability study

DOI

https://doi.org/10.22376/ijpbs.v16i4.126

References

1. Hassan HA, Ahmed HS, Hassan DF. Free radicals and oxidative stress: Mechanisms and therapeutic targets. Human Antibodies. 2024 Nov;32(4):151-67.

2. Sen AK, Pandey H, Maheshwari RA, Zanwar AS, Velmurugan R, Sen DB. Novel UV spectroscopic methods for simultaneous assessment of empagliflozin, linagliptin and metformin in ternary mixture. Indian J Pharm Educ Res. 2022 Oct 1;56(4s):s669-81.

3. Konari SN, Jacob JT, Prakash V. Stability indicating UV spectrophotometric method for linagliptin and metformin in pharmaceutical dosage form. Pharm Methods. 2017 Jul 1;8(2):121-6.

4. Liu J, He J, Huang Y, Hu Z. Resveratrol has an overall neuroprotective role in ischemic stroke: a meta-analysis in rodents. Frontiers in Pharmacology. 2021 Dec 20;12:795409.

5. Kaur A, Tiwari R, Tiwari G, Ramachandran V. Resveratrol: A vital therapeutic agent with multiple health benefits. Drug research. 2022 Jan;72(01):5-17.

6. Shayganfard M. Molecular and biological functions of resveratrol in psychiatric disorders: a review of recent evidence. Cell & bioscience. 2020 Nov 7;10(1):128.

7. Jain R, Jain N, Jain DK, Patel VK, Rajak H, Jain SK. Novel UV spectrophotometer methods for quantitative estimation of metronidazole and furazolidone using mixed hydrotropy solubilization. Arabian Journal of Chemistry. 2017 Feb 1;10(2):151-6.

8. Lotfy HM. Absorbance subtraction and amplitude modulation as novel spectrophotometric methods for the analysis of binary mixtures. Int. J. Pharm. Pharm. Sci. 2014;6(1):735-41.

9. Tayde MA, Patil RA, Katti SA, Pawar SH. Development and validation of HPTLC and first derivative spectrophotometric methods for determination of levetiracetam in bulk and tablet dosage form. Research Journal of Pharmacy and Technology. 2024 Mar 1;17(3):979-85.

10. McMahon ME, Abbott A, Babayan Y, Carhart J, Chen CW, Debie E, Fu M, Hoaglund-Hyzer C, Lennard A, Li H, Mazzeo T. Considerations for updates to ICH Q1 and Q5C stability guidelines: embracing current technology and risk assessment strategies. The AAPS journal. 2021 Sep 16;23(6):107.

11. Guideline IH. Validation of analytical procedures Q2 (R2). ICH: Geneva, Switzerland. 2022;1.

12. Majithia RH, Khodadiya A, Patel VB. Spectrophotometric method development and validation for simultaneous estimation of Anagliptin and Metformin HCl BY Q-Absorption ratio method in synthetic mixture. Heliyon. 2020 May 1;6(5).

13. Ali A, Sultana N, Qadir A, Waheed A, Mujeeb M, Sultana Y, Aqil M. Development and validation of UV-spectroscopy based stability indicating method for simultaneous determination of fluoxetine hydrochloride and embelin.

14. Sultana N, Ali A, Waheed A, Aqil M, Sultana Y, Mujeeb M, Qadir A. Development And Validation of UV-Spectroscopy Based Stability Indicating Method For Simultaneous Estimation of Risedronate Sodium AndUrsolic Acid. World J Pharm Res. 2022 Feb 7;11:1293-306.