Invitro antimicrobial activity of bitter melon leaf extract using well diffusion method

Abstract

The aim of present study examines the invitro antimicrobial properties of leaf removes from Momordica charantia, commonly known as bitter melon. Utilizing different extraction techniques, we arranged separates and considered their viability in contrast to a scope of pathogenic microorganisms, including microbes and growths. Using a paper plate dispersion test, its antimicrobial viability against Staphylococcus aureus, Salmonella typhi, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa was assessed using its methanolic extracts. The Agar Well-Dispersion Strategy ensured adequate antifungal efficacy against specific strains of Penicillium and Aspergillus. The base inhibitory focus (MIC) was determined using the Stock Weakening method and the Agar Well-Dispersion method. Antimicrobial examines, including agar dissemination and least inhibitory fixation (MIC) tests, were directed to decide the viability of the concentrates. The outcomes exhibited huge antimicrobial properties, with fluctuating levels of hindrance across various microbial strains. These discoveries propose that Momordicacharantia leaf separates have likely remedial applications as normal antimicrobial specialists, featuring the requirement for additional investigation into their dynamic mixtures and instruments of activity. This study contributes to the growing interest in plant-derived substances for combating microbial resistance.