Combined effect of Justicia adhatoda and Crocus sativus on Zebrafish Model for Cognitive enhancement

Abstract

Brain-related problems can include brain injuries, brain tumors, and brain diseases. They can also include mental health disorders such as Depression, Anxiety, Bipolar disorder, Post- traumatic stress disorder (PTSD). This study explores the combined cognitive-enhancing potential of Justicia adhatoda and Crocus sativus using a zebrafish (Danio rerio) model. Both plants are known for their neuroprotective and mood-stabilizing properties. Methanolic extracts were prepared and tested through behavioral assays, including the Open Field Test (OFT) and Novel Object Recognition Test (NORT), which assess anxiety, locomotor activity, and memory function. Results indicated that the combination group exhibited significantly higher central zone activity and recognition index compared to the control and individual treatment groups. Justicia adhatoda enhanced cognitive performance through cholinergic modulation and antioxidant effects, while Crocus sativus acted via serotonergic pathways and BDNF upregulation. Their synergistic interaction led to improved memory, reduced anxiety-like behavior, and better cognitive processing. This study highlights the therapeutic potential of combining these plant extracts for CNS enhancement and supports their use as a natural, multi-targeted alternative for managing cognitive disorders. Further research is recommended to validate these findings in clinical settings.