Comprehensive Phytochemical Isolation and in Vitro Biological Assessment of Pimpinella heyneana: a Promising Underexplored Medicinal Herb

Saritha Kodithala; Himaja Boreddy; Nandini T; Karunya K; Mohammad Mansoor; Sampath Kumar

Authors

Saritha Kodithala Associate Professor, HOD, Department of Pharmacognosy, Vignan Institute of Pharmaceutical Sciences, Near Ramoji filmcity, Yadadri Bhuvanagiri Dist, Telangana.
Himaja Boreddy Assistant Professor, Department of Pharmacology, Vignan Institute of Pharmaceutical Sciences, Near Ramoji filmcity, Yadadri Bhuvanagiri Dist, Telangana
T.Nandini Assistant Professor, Bojjam Narasimhulu College of Pharmacy, Department of Pharmacology, Hyderabad.
K. Karunya Assistant Professor, Bojjam Narasimhulu College of Pharmacy, Department Of Pharmacology, Hyderabad.
Mohammad Mansoor Professor, Dept of Pharmacology, Devaki Amma Memorial College of Pharmacy, Kerala.
Sampath Kumar Associate Professor, Department of Pharmacology, Jyotismathi Institute of Pharmaceutical Sciences, Karimnagar, Telangana.

Abstract

The present study investigates the phytochemical profile of Pimpinella heyneana with emphasis on isolation, structural characterization, and biological evaluation of its bioactive constituents. Ethanolic extract was prepared and subjected to chromatographic separation. Structural elucidation was performed using FTIRspectrometry and HPLC chromatography. The extract showed majorly the presence of flavonoids and phenolic compounds. In Vitro evaluation revealed significant antioxidant (IC₅₀: 42.6 µg/mL), antimicrobial activities. HPLC analysis confirmed multiple phytoconstituents with a major peak at 5.84 min. These findings support the therapeutic potential of P. heyneana.

Keywords:

Pimpinella heyneana, phytochemicals, HPLC, FTIR, antioxidant, antimicrobial

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Comprehensive Phytochemical Isolation and in Vitro Biological Assessment of Pimpinella heyneana: a Promising Underexplored Medicinal Herb

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