Bioproduction of exopolysaccharide by agrobacterium tumefaciens using date molasses and their potential applications
Abstract
The present study aimed to optimize the production of exopolysaccharides (EPS) by Agrobacterium tumefaciens using dates molasses (DM) as a carbon source. Various culture conditions, seed culture, inoculum size, and nitrogen supplements were tested to achieve maximum bacterial growth and EPS yield.After optimizing the media and cultural conditions, the EPS was purified by gas-liquid chromatography. The purified EPS was characterized for its functional properties, including sugar composition, viscosity, emulsification, and serological properties.The results indicated that DM has a high percentage of sugars (55.77%), with potassium as the main element (60.40%). A. tumefaciens produced a single EPS at 96 hours of fermentation under static conditions at 30 °C, 3 to 10% DM, 7 g/L KNO3, and 36 h of seed culture. The succinic acid in the basal medium and inducing agents such as ribose, glucose, and sucrose stimulated the growth and EPS yield of the bacterium. D- glucose was observed to be the main component in the purified EPS 100% glucose. The viscosity property increased with EPS concentration and showed a reversible behavior as the temperature rose from 10- 100 oC.An emulsification of 45% was obtained in the filtrate containing the EPS. The antisera against crude EPS showed activity in the double immunodiffusion test, indicating that the crude acetone-precipitated polymer was immunogenic. However, the pure polymer did not show any precipitation. Pure EPS is non-toxic, emulsifying, and non-immunogenic, making it suitable for blood plasma expander or food additive.
Keywords:
Agrobacterium tumefaciens, Exopolysaccharide, date molasses, bacterial polysaccharide, emulsifierDOI
https://doi.org/10.22376/ijpbs.v16i2.79References
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