Molecular Phylogeny, and Integrated Nutritional, Antioxidant, Antidiabetic, and Anticancer Assessment of Littoraria scabra from Coringa Mangroves
Abstract
The present study investigates the nutritional composition, antioxidant, antidiabetic, and anticancer properties of the estuarine gastropod Littoraria scabra, collected from the Coringa mangroves of Andhra Pradesh, India. Molecular identification using the mitochondrial cytochrome oxidase subunit I gene confirmed species identity and revealed a close phylogenetic relationship with other L. scabra isolates, forming a distinct clade within the genus. Nutritional analysis of the muscle tissue showed high moisture (76.87±5.68 g/100 g FW) and protein content (17.83±2.56 g/100 g FW), with moderate levels of ash, carbohydrates, lipids, and free amino acids. Methanolic extracts of L. scabra muscle exhibited concentration-dependent antioxidant activities, as demonstrated by increasing free radical scavenging capacities in DPPH, ABTS, FRAP, superoxide, and nitric oxide assays. The extracts also demonstrated significant in vitro antidiabetic potential, including enhanced glucose adsorption, inhibition of hemoglobin glycosylation, increased glucose uptake by yeast, and α-amylase inhibition, all with favourable EC₅₀ values. Furthermore, the methanolic extracts showed dose-dependent cytotoxicity against MCF-7 and HL-60 cell lines, with IC₅₀ values of 6.91 mg and 10.26 mg, respectively. These findings highlight the nutritional value of L. scabra and demonstrate its promising bioactive properties, supporting its potential as a source of natural antioxidants, antidiabetic agents, and anticancer compounds. This study contributes valuable insights for future research on estuarine gastropods as alternative food sources and reservoirs of therapeutic compounds.
Keywords:
Littoraria scabra, nutritional value, antioxidant activity, antidiabetic activity, anticancer activityDOI
https://doi.org/10.70604/ijzels.v3i2.198References
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